منابع مشابه
Uukuniemi virus contains an RNA polymerase.
An RNA-dependent RNA polymerase activity has been found associated with Uukuniemi virions. The enzyme activity is expressed only after disrupting the virions with the nonionic detergent Triton X-100 and is absolutely dependent on Mn2+, whereas Mg2+ is not required, a finding that distinguishes this polymerase from those of other enveloped minus-strand RNA viruses. Within the range pH 7.2 to 8.5...
متن کاملUukuniemi Virus as a Tick-Borne Virus Model
UNLABELLED In the last decade, novel tick-borne pathogenic phleboviruses in the family Bunyaviridae, all closely related to Uukuniemi virus (UUKV), have emerged on different continents. To reproduce the tick-mammal switch in vitro, we first established a reverse genetics system to rescue UUKV with a genome close to that of the authentic virus isolated from the Ixodes ricinus tick reservoir. The...
متن کاملInsights into bunyavirus architecture from electron cryotomography of Uukuniemi virus.
Bunyaviridae is a large family of viruses that have gained attention as "emerging viruses" because many members cause serious disease in humans, with an increasing number of outbreaks. These negative-strand RNA viruses possess a membrane envelope covered by glycoproteins. The virions are pleiomorphic and thus have not been amenable to structural characterization using common techniques that inv...
متن کاملPosttranslational processing of Uukuniemi virus glycoproteins G1 and G2.
Uukuniemi virus, which matures specifically in the Golgi complex, contains two species of envelope glycoproteins, G1 (Mr, 70,000) and G2 (Mr, 65,000). These are translated as a polyprotein, p110, from an mRNA which is complementary to the medium-sized segment of the virion RNAs. By synchronized initiation of protein synthesis and pulse-labeling, it was shown that glycoprotein G1 is amino termin...
متن کاملMonosaccharide sequence of protein-bound glycans of Uukuniemi virus.
Uukuniemi virus, a member of the Bunyaviridae family, was grown in BHK-21 cells in the presence of [(3)H]mannose. The purified virions were disrupted with sodium dodecyl sulfate and digested with pronase. The [(3)H]mannose-labeled glycopeptides of the mixture of the two envelope glycoproteins G1 and G2 were characterized by degrading the glycans with specific exo-and endoglycosidases, by chemic...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1975
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.16.5.1296-1307.1975